The technique of fine-needle aspiration of palpable mass lesions of the head and neck

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      This article describes the technique of fine-needle aspiration biopsy in a stepwise fashion. The aspiration procedure is broken down into three phases: The preparatory phase, which includes patient counseling, preparation of equipment and materials, and patient positioning; the actual aspiration; and the specimen handling phase, which includes fixation and smear-making. Indications for the procedure, equipment required, ancillary techniques, and potential complications are also briefly discussed. Although the techniques presented are applicable to palpable masses at any anatomic site, the discussion is tailored to the head and neck area.


        • Frable WJ
        Thin-needle aspiration biopsy.
        in: Bennington J Major Problems in Pathology. Vol. 14. Saunders, Philadelphia, PA1983
        • Ljung BM
        Principles of aspiration biopsy: Techniques of aspiration and smear preparation.
        in: Koss LG Woyke S Olszewski W Aspiration Biopsy: Cytologic Interpretation and Histologic Basis. 2nd ed. Igaku-Shoin, New York, NY1992
        • Stanley MW
        • Lowhagen T
        Fine Needle Aspiration of Palpable Masses.
        Butterworth-Heinemann, Boston, MA1993
        • DeMay RM
        The Art and Science of Cytopathology.
        ASCP Press, Chicago, IL1996
        • Powers CN
        • Frable WJ
        Fine Needle Aspiration of Biopsy of the Head and Neck.
        Butterworth-Heinemann, Boston, MA1996
        • Frable MAS
        • Frable WJ
        Fine needle aspiration biopsy of salivary glands.
        Laryngoscope. 1991; 101: 245-249
        • Abele JS
        • Miller TR
        • King EB
        • et al.
        Smearing techniques for the concentration of particles from fine needle aspiration biopsy.
        Diagn Cytopathol. 1985; 1: 59-65
        • Chan JKC
        • King ITM
        Rehydration of air-dried smears with normal saline: Application in fine-needle aspiration cytologic examination.
        Am J Clin Pathol. 1988; 89: 30-34
        • Kennerdell JS
        • Slamovits TC
        • Dekker A
        • et al.
        Orbital fine-needle aspiration biopsy.
        Am J Ophthalmol. 1985; 99: 547-551
        • Zajdela A
        • Zillhardt P
        • Voillemot N
        Cytological diagnosis by fine needle sampling without aspiration.
        Cancer. 1987; 59: 1201-1205
        • Panuzi C
        • Paliotta DS
        • Papini E
        • et al.
        Cutaneous seeding of a follicular thyroid cancer after fine-needle aspiration biopsy?.
        Diagn Cytopathol. 1994; 10: 156-158
        • Hales MS
        • Hsu FSF
        Needle tract implantation of papillary carcinoma of the thyroid following aspiration biopsy.
        Acta Cytol. 1990; 34: 801-804
        • Engzell V
        • Franzen S
        • Zajicek J
        Aspiration biopsy of tumors of the neck. II. Cytologic findings in 13 cases of carotid body tumor.
        Acta Cytol. 1971; 15: 25-30
        • Koss LG
        • Woyke S
        • Olszewski W
        Aspiration Biopsy: Cytologic Interpretation and Histologic Basis.
        2nd ed. Igaku-Shoin, New York, NY1992
        • Fleming MV
        • Oertel YC
        • Rodriguez ER
        • et al.
        Fine-needle aspiration of six carotid body paragangliomas.
        Diagn Cytopathol. 1993; 9: 510-515